Tools to help you designing your experiment with our BD X20 Fortessa

Two sites that display spectral properties of many fluorochromes:
Link to Invitrogen Fluorescence Spectra Viewer
Link to BD Fluorescence Spectrum Viewer

Our solution

It is a simple, but still efficient solution. It will help you to check whether the combination that you intend to use is correct. It works online, just follow the following steps:

1) Select the fluorochrome that you intend to use
2) Fill the 'Antibody' field (for instance Anti-mCD3)
3) If you are able to enter your combination, this means that your combination is correct, simple, isn't it?

Last update: 28 March 2019 - Correction of the wavelength of the UV laser: 355nm (instead of 375nm)

N°
Lasers
Antibody
Fluorochrome
Band Pass
Detector A
Blue (488nm)
780/60
Detector B
Blue (488nm)
710/50
Detector C
Blue (488nm)
610/20
Detector D
Blue (488nm)
575/26
Detector E
Blue (488nm)
530/30
Detector A
Red (633nm)
780/60
Detector B
Red (633nm)
730/45
Detector C
Red (633nm)
670/30
Detector A
Violet (405nm)
780/60
Detector B
Violet (405nm)
710/50
Detector C
Violet (405nm)
670/30
Detector D
Violet (405nm)
610/20
Detector E
Violet (405nm)
525/50
Detector F
Violet (405nm)
450/50
Detector A
UV (355nm)
820/60
Detector B
UV (355nm)
740/35
Detector C UV (355nm)
515/30
Detector D
UV (355nm)
379/28


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